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M94A3308.TXT
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1994-10-25
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Document 3308
DOCN M94A3308
TI Analysis of pol gene of a non-infectious HIV-1 clone.
DT 9412
AU Nakano T; Sano K; Goto T; Morimatsu S; Ueda S; Ikuta K; Kato S; Nakai M;
Department of Microbiology, Osaka Medical College, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0043). Unique
Identifier : AIDSLINE ICA10/94369267
AB OBJECTIVE: We analyzed the nucleotide sequence of pol gene encoding
protease (PR) in a non-infectious HIV-1 producing cell clone L-2, and
examined the expression of pol gene products. METHODS: Conventional
dideoxy-termination method was used to sequence PR coding region of L-2
and Molt-4/LAV-1 cells. The expressions of PR, reverse transcriptase
(RT), and integrase (IN) in these cells were investigated with IF test
using specific antibodies. RESULTS: There was insertion mutation at the
42nd base of PR coding region of L-2. A stop codon was made at the
position of the 30th codon of PR. The expressions of PR, RT, and IN in
L-2 cell were not detectable. DISCUSSIONS AND CONCLUSIONS: Since L-2
lacked PR, it was considered that the intact gag polyprotein in released
particles was not cleaved. We found a pol-unexpressing wild mutant of
cultured cells, and the mutant may be useful to analyze further
mechanism of the onset of AIDS in vivo.
DE Acquired Immunodeficiency Syndrome/ETIOLOGY Cell Line Cloning,
Molecular DNA Nucleotidyltransferases/GENETICS Gene Expression Gene
Products, gag/GENETICS *Genes, pol Human
HIV-1/*GENETICS/PATHOGENICITY Mutation Reverse Transcriptase/GENETICS
Virulence/GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).